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Description:Here, we adopted serial block-face SEM imaging, a powerful tool for analyzing the 3D cellular ultrastructure, to precisely reveal the morphological process of podocyte development, such as the formation of foot processes. Morphological alteration of podocytes vigorously progresses, especially from the S-shaped body to maturing glomerulus stages, as shown in Fig. RESULTS Glomerulus development is morphologically divided into seven stages in the mammalian metanephric kidney, including condensation, renal vesicular, comma-shaped body, S-shaped body, capillary loop, maturing glomerulus and mature stages Reeves et al. Order her custom videos at www. We thus examined the podocytes during this period. Immature podocytes were columnar in shape and connected to each other by the junctional complex, which migrated toward the basal side of the cell.